| Carlee M. Cunningham, a senior PhD student in the Habas Lab recently published a paper with Dr. Ray Habas, Dr. Gianfranco Bellipanni and Dr. Darius Balciunas entitled "Deletion of morpholino binding sites (DeMOBS) to assess specificity of morphant phenotypes". The study addresses problems associated with antisense Morpholino Oligomer use in embryonic zebrafish as a knock-down technology. Using a combination of CRISPR/Cas9 and Morpholino technologies, the study was able to provide evidence that introducing mutations into Morpholino binding sites may be a valuable method in teasing apart the differences between genuine morphant phenotypes and those caused by toxicity or off-target effects. The paper can be found here. |  |
