Carlee M. Cunningham, a senior PhD student in the Habas Lab recently published a paper with Dr. Ray Habas, Dr. Gianfranco Bellipanni and Dr. Darius Balciunas entitled "Deletion of morpholino binding sites (DeMOBS) to assess specificity of morphant phenotypes". The study addresses problems associated with antisense Morpholino Oligomer use in embryonic zebrafish as a knock-down technology. Using a combination of CRISPR/Cas9 and Morpholino technologies, the study was able to provide evidence that introducing mutations into Morpholino binding sites may be a valuable method in teasing apart the differences between genuine morphant phenotypes and those caused by toxicity or off-target effects.
The paper can be found here.
Jaylene Everett was an undergraduate researcher in the Habas Lab for several semesters. While with us, Jaylene assisted one of our graduate students in understanding the interaction between Custos and Daple.
Jaylene just completed her degree earning a Bachelor of Science in Biology. She will continue on to attend Lake Erie College of Osteopathic Medicine starting in July.
Fun fact: Jaylene was also on the gymnastics team at Temple!
Congrats Jaylene, we are so proud of you!
Dr. Ray Habas and a former Habas Lab postdoc, Dr. Yuko Komiya, were recently involved in co-authoring a newly published paper in the open-access journal PLOS One. Dr. Habas and Dr. Komiya accompanied first author Dr. Masa-aki Nakaya and principal investigator Dr. Reiko Ajima, both at Yokohama City University Graduate School of Medical Science, Yokohama, Kanagawa, Japan. Both Dr. Nakaya and Dr. Ajima have track records publishing research on Wnt signaling, embryonic development, planar cell polarity and the Daams.
The paper, entitled "Placental Defects Lead to Embryonic Lethality in Mice Lacking the Formin and PCP Proteins Daam1 and Daam2" examines the relationship between murine Daam1, Daam2 and Wnt5a in mouse embryonic development, specifically investigating the role of Daam1 and Daam2 in development of placental vasculature.
The paper can be found here.
Amulya Surakanti is an undergraduate researcher in the Habas Lab and has been with us since her freshman year in 2017. For the entire month of May Amulya was in Kodaikanal, India where she volunteered with Foundation for International Medical Relief of Children (FIMRC).
Here is what she had to say about the program and her experience:
"FIMRC [is] a non-profit organization that wants to increase access to healthcare in third world countries. One of the 9 project sites is in Kodaikanal, India. Kodaikanal is one of the most diverse towns in India and is considered a tourist spot for the locals. Some of the projects that FIMRC launched in Kodaikanal are community surveys to track the health of families in different communities, the health and nutrition programs at the creches, which are day centers for low-income families, and health education sessions for the communities. In addition, FIMRC field operations director, Arun Selvaraj, recently started a program which involves compost bins for families to dispose their trash, but later use it as compost for gardening. There is a FIMRC clinic, which provides primary care for the underprivileged families. The volunteers also get to go to three different hospitals: KHMS, Van Allen Hospital and the Government Hospital. They all have different clinical setting and provide care at different levels. Van Allen Hospital also runs a mobile clinic camp couple times a week, which serves villages surrounding Kodaikanal that do not have access to hospitals or healthcare in their village. The volunteers participate in the mobile clinic program as well and learn about the health situation in various communities."
Keep up the amazing work Amulya!